IRWIN FRIDOVICH. The Department of Biochemistry, Duke University Medical Center,. Durham, North Carolina Received April 21, Superoxide. Beauchamp, C. and Fridovich, I. () Superoxide Dismutase Improved Assays and an Assay Applicable to Acrylamide Gels. Analytical Biochemistry, Author: Beauchamp C, Journal: Analytical biochemistry[/11] Fridovich I. Find all citations in Analytical Biochemistry [01 Nov , 44(1)]. /.

Author: Mazudal Shaktikora
Country: Ecuador
Language: English (Spanish)
Genre: Video
Published (Last): 2 July 2015
Pages: 341
PDF File Size: 14.6 Mb
ePub File Size: 5.74 Mb
ISBN: 182-5-89870-403-4
Downloads: 94809
Price: Free* [*Free Regsitration Required]
Uploader: Tuzuru

No difference in the quantitative reliability was found in any riboflavin concentration.

Animals were sacrificed at the end of treatment, testes were collected and weighed. This technique has already been employed to analyze SOD protein expression levels in extracts of Escherichia coli Bertrand et al.

Glutathione peroxidase, superoxide dismutase and catalase activities in anr with chronic hepatitis. Low micromolar to low millimolar concentrations of yellow riboflavin can be mixed with the blue of reduced nitroblue tetrazolium to controllably produce blue, purple, yellow-brown, or yellow gel backgrounds.

SOD research in medicine and biochemistry has warranted the development of multiple assay variants to overcome specific experimental constraints or to combine the SOD assay with other enzyme assays. Budi Handono, Firman F. Herein we describe a straightforward technique to reliably adjust the background color of polyacrylamide gels without compromising assay efficacy.


Treatment with single doses of these agents time dependently increased testicular MDA, decreased Beaucahmp level and induced abnormal testicular fridogich changes. Nagwa Abdallah Ismail, Sawsan H. Yan Li, Nathaniel P.

There was a problem providing the content you requested

Fine-tuning reagent concentrations to effectively visualize bands continue to be a major research obstacle in assay development.

Quantitative reliability of these alternative stains was assessed by plotting determined band intensity values against known enzyme loads. The concurrent use of these drugs may place more adverse burden on testicular function because reports have associated these drugs individually with decreased ans function.

Mose, Tri Hanggono Achmad. Testicular levels of malondialdehyde MDAsuper oxide dismutase SOD and histopathological changes were also analyzed. No significant frieovich effects were observed in all evaluated parameters when these agents were co-administered. The reduction of nitroblue tetrazolium by superoxide radicals generated from photo-reactive riboflavin has been in use for more than four decades to detect superoxide dismutase Fridivich on nondenaturing polyacrylamide gels.

Scientific Research An Academic Publisher.

Sperm count, sperm motility, and morphology were evaluated. The advantage of this technique is that the assay is not modified by the introduction of new reagents.


Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.

Assay sensitivity was assessed by comparing lowest possible frisovich enzyme load of the experimental stains with the 0.

The correlation R2 values of trial averages were compared against the average correlation of the standard 0.

Okasha, Anil Dhawan, Azza M. Analytical Biochemistry, 44, Advances in Enzyme ResearchVol. Due to lack of significant synergistic effects on all evaluated parameters with the co-administration of these agents, the concurrent use of these agents in the management of HIV and co- infections may not have any deleterious effect on male reproductive function.

Eighty 80 adult male rats which were divided into five 5 groups A-E were used in this study. The minimum reliable sensitivity of beauchxmp assay was found to be 10 ng for each concentration of riboflavin.