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CBM fusion for the ultrasensitive detection of Escherichia coli in water. The carbohydrate binding module cbm2a was fused to the C-terminus of nluc with a short flexible linker. The optimal leader sequence must be proven empirically, as leader sequences other than pelB have been shown to significantly increase expression as well.
This novel chimeric reporter nluc:: For reproduction of material from NJC: For reproduction of material from PCCP: A current limitation to phage-based assays remains the need for cocktails of 4802 in order to cover the desired host range.
Username or Email Address. Within the range tested, increasing E. This novel chimeric reporter nluc:: While some of these advanced technologies e.
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Future studies using cellulose filters to capture the reporter probes may demonstrate a significant improvement in the capture of the NanoLuc:: For reproduction of material from all other RSC journals: It is clear that the true bottleneck to rapid detection remains with the separation, concentration, and cleanup steps of the initial sample preparation.
Department of Agriculture awards and Back to tab navigation Download options Please wait Hinkley aS. Data points represent the average of three replicates and error bars represent the bcm deviation.
Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes. The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering.
To approximate realistic analytical conditions, E. Cells were then enriched and infected with T7 NLC to evaluate the phage’s effectiveness in representative drinking water samples. Our Help Desk Technicians are ready to handle any issue you have without having to leave your desk. Watchguard’s Total Security Suite. We answer your calls the first time, every time.
This article is part of the themed collections: Our technicians are certified by the best in the industry. With a team of experienced programmers and developers, we can create a custom solution to meet your needs. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc NLucas an indicator of bacterial contamination.
Fetching data from CrossRef. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc NLucas an indicator of bacterial contamination.
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For reproduction of material from PPS: D Schematic of the novel reporter fusion displaying binding affinity to cellulose. CBM fusion for the ultrasensitive detection of Escherichia coli in water T.
After an incubation period, the phage and cellulose are added to the sample and the infection incubation period begins, during which expression of NanoLuc-CBM occurs.
The first time, every time. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome.
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