CHARDAKOV METHOD PDF
2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.
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If you wish to download it, please recommend it to your friends in any social system. One distinct advantage of this technique is that it charvakov a more accurate estimate of water potential. Draw the best fit line for your data.
In both techniques, tissue samples are incubated in a series of solutions of known osmotic water potential. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated.
Weigh the cores to the nearest 0. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.
The solution gains or looses water depending on the water potential of the tissue. This increases the tissue weight and may lead to small errors. Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Cell Transport Metuod movement of molecules can be either passive no energy or active needs energy depending upon the membrane structure and concentration.
Does it correspond to the value obtained by the Chardakov technique?
Examine the cores as you weigh them. The water potential measured by this chsrdakov should be the same as that obtained the Chardakov method. Which is more accurate? In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes.
About project SlidePlayer Terms of Service. Periodically swirl the containers. Be sure not to include any fragments of the skin. Cyardakov method do you think will be more accurate?
OK Any substance that can change shape or flow easily.
Repeat for all solutions. Pour off the solutions into a set of empty, correspondingly labeled tubes. My presentations Profile Feedback Log out. Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, disperses, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly. Record your results Table 2 and repeat this procedure for each of the sucrose solutions. Change in Weight final – initial g.
Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference. A measure of how much material is packed into a unit volume of the material The fewer. Dispense 10 mL of water or a sucrose solution 0. A “kink” may be observed in the graph below the x-axis. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.
Change in weight of potato cores incubated in sucrose solutions. Cytology Osmosis and Water relations.
What is the water potential calculated by this method? This technique for measuring water potential is similar in theory to the Chardakov method and shares the advantage of being simple to perform and doesn’t require expensive equipment.
Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix. The water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i.
Incubate the cores for 1. In this method, tissue samples are weighed before and after incubation in a series of solutions of known osmotic water potential.
Investigating osmosis: measuring the water potential of a potato cell
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