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Membranes were blocked for western blots, as described previously and primary antibody immune-reactive complexes were identified using alkaline phosphatase-conjugated secondary antisera Sigma Aldrich with enzyme-linked chemifluorescence GE Healthcare, Piscataway NJ and quantified with a Typhoon phosphorimager. Figure S2 AMI-mediated alteration of synaptic factors and neuroprotective signaling in extended-culture primary hippocampal and tornincassa cells.


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