The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or . objetivos: conocer las diferentes técnicas de aglutinación usadas en el laboratorio. adquirir habilidad y destrezas en las técnicas de. Prozone effect: No prozone effect was detected upon mg/dL. 4. . Efecto prozona: No se observa efecto prozona hasta valores de

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The Clinical Biochemist Reviews.

“efecto prozona” in English

Retrieved from ” https: In this case, free antigen prosona in competition with captured antigen for detection antibody binding. Views Read Edit View history. However, when the serum is diluted, the blocking antibody is as well and its concentration decreases enough for the proper precipitation reaction to occur. Retrieved 3 February By using this site, you agree to the Terms of Use and Privacy Policy.

efecto prozona – English translation – Spanish-English dictionary

Has its time arrived? Because no agglutination occurs, the test is interpreted as negative.


If the antibodies agglutinate with efdcto antigen to form immune complexesthen the test is interpreted as positive. Thus the proper precipitation reaction does not take place.

Term Bank – efecto prozona – Spanish English Dictionary

In an agglutination test, eecto person’s serum which contains antibodies is added to a test tubewhich contains a particular antigen. The serological test is mainly seen in the precipitation reaction.

The antibody that fails to react is known as the blocking antibody and prevents the precipitating antibody from binding to the antigens.

The effect can also occur because of antigen excess, when both the capture and detection antibodies become saturated by the high analyte concentration. However, if too many antibodies are present that can bind to the antigen, then the antigenic sites are coated by antibodies, and few or no antibodies directed toward the pathogen are able to bind more than one antigenic particle.

This page was last edited on 14 Augustat In this case, the result is a false negative. From Wikipedia, the free encyclopedia.

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The range of relatively high antibody concentrations within which no reaction occurs is called the prozone. Examples include high levels of syphilis antibodies in HIV patients or high levels of cryptococcal antigen leading to false negative tests in undiluted samples.

In this case, no sandwich can be formed by the egecto antibody, the antigen and the detection antibody.

The hook effect or the prozone prozoja is a type of interference which plagues certain immunoassays and nephelometric assaysresulting in false negatives or inaccurately low results. The phenomenon is caused by very high concentrations of a particular analyte or antibody and is most prevalent in one-step sandwich immunoassays.

Other common forms of interference include antibody interference, cross-reactivity and signal interference.