JCM 5046 PDF

JCM 5046 PDF

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Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Martinsa Adriana G.

Journal List Antimicrob Agents Chemother v. We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable.

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S, sensitive; R, resistant. During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure.

For comparison purposes, the first reported BKC-producing strains, i. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. Only 2 BKCproducing K. Author information Article jc, Copyright and License information Disclaimer.

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Prepublished online May The bla BKC-1 gene was inserted into a kb plasmid that hcm identical to the previously reported plasmid, p Sampaiob, c and Ana C. Please review our privacy policy.

Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

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To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates.

A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions. These carbapenem resistance genes are frequently harbored on mobile genetic elements that are highly transmissible, contributing to the increasing frequency of carbapenem-resistant Enterobacteriaceae 1.

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J Antimicrob Chemother The Kpn and KpnJ strains showed different plasmid profiles. Among the Klebsiella strains, The other authors have no conflicts to declare. Antimicrob Agents Chemother J Clin Microbiol Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance.

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Support Center Support Center. Open in a separate window. Plasmid extraction of K. The principal objective of this study was to evaluate the frequency of bla BKC-1 by testing a collection of Klebsiella isolates. Clinical and Jvm Standards Institute. Initially, we performed transformation by electroporation into Escherichia coli Top The carbapenemase was encoded by bla BKC-1which was located on a small nonconjugative plasmid 2. Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of jjcm resistance phenotype.

BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K.